Shin WJ, Son YJ, Kang KJ, Han HS, Cho KC, Son GC (2008) Trichosanthes kirilowii tuber extract induces G2/M phase arrest via inhibition of tubulin polymerization in HepG2 cells. Ryu SY, Lee SH, Choi SU, Lee CO, No Z, Ahn JW (1994) Antitumor activity of Trichosanthes kirilowii. Rout GR (2006) Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis (L.) Kuntze and associated biochemical changes. Quoirin M, Lepoivre P (1977) Improved medium for in vitro culture of Prunus sp. Qian LC, Qi HX, Yang HJ, Zhang JM (2012) Molecular phylogeny of Chinese snakegourd ( Trichosanthes kirilowii Maxim) based on cytological and AFLP analyses. Pillai GS, Martin G, Raghu AV, Lyric PS, Balachandran I, Ravindran PN (2008) Optimizing culture conditions for in vitro propagation of Trichosanthes cucumerina L.: an important medicinal plant. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains.
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Lucas WJ, Groover A, Lichtenberger R, Furuta K, Yadav SR, Helariutta Y, He XQ, Fukuda H, Kang J, Brady SM, Patrick JW, Sperry J, Yoshida A, L’opez-Mill’an AF, Grusak MA, Kachroo P (2013) The plant vascular system: evolution, development and functions. Lloyd G, MCCown B (1980) Commercially-feasible micro-propagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Linsmaier EM, Skoog F (1975) Organic growth factor requirements of tobacco. Kim HK, Moon CB, Kang YM, Choi GY, Ji UY, Ju SY, Seo YB, Choo KB (2015) Korean medicinal materials, vol 2. Kang Y, Lee Y, Choi J, Komakech R, Min J, Ju S, Kim WS, Youne C, Kim GY, Moon CB (2018) Maximizing seedling and root tuber production in Polygonum multiflorum for use in ethnomedicine. Gamborg OL, Miller AR, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Sci Sin B 18:659–668ĭe Greef W, Jacobs M (1979) In vitro culture of sugar beet: description of a cell line with high regeneration capacity. Ĭhu CC, Wang CC, Sun CS, Hsu C, Yin CK, Chu YC, Bi YF (1975) Establishment of efficient medium for another culture of rice through comparative experiments on the nitrogen sources. J Am Soc Hortic Sci 109:343–347Ĭhen Y, Miao Y, Huang L, Li J, Sun H, Zhao Y, Yang J, Zhou W (2014) Antioxidant activities of saponins extracted from Radix Trichosanthis: an in vivo and in vitro evaluation. J Plant Sci 3:24–32Īnderson WC (1984) A revised tissue culture medium for shoot multiplication of rhododendron. kirilowii.Īgarwal M (2015) Tissue culture of Momordica charantia L.: a review. Therefore, these findings provide a basis for future large-scale in vitro propagation of T. The micropropagated plants that were acclimatized for 8 wk in the greenhouse produced mature root tubers after planted in the field for 3 mo. At 5 wk, De Greef and Jacobs medium supplemented with 0.1 mg L −1 kinetin led to optimal shoot growth, while the same medium supplemented with 0.5 mg L −1 indole 3-butyric acid induced optimal root growth, also at 5 wk. Ten different media supplemented with different concentrations of plant growth regulators were evaluated. The purpose of this study was to develop a standard protocol for the in vitro micropropagation of T. This coupled with uncertainty in identification of the plant when collected from the wild calls for a standard in vitro propagation system to meet the increasing demand for it. Roots harvested for medicinal use take over 3 yr to mature when the plant is grown in a traditional way through cultivation in the field.
Its roots, fruits, and seeds are used as food and medicine. is a vital traditional herbal medicinal plant found in northeastern Asia.
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